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In vitro cytotoxicity of ibipinabant against: ( A ) Vero and ( B ) ME-180 cells. Results are shown as a percentage of cell viability relative to the negative control (DMSO). ( C ) Hemolytic activity of ibipinabant against human <t>RBCs.</t> The results are shown as a percentage of RBC hemolysis for each concentration of ibipinabant relative to 0.1% Triton X-100 (TX-100; positive control with complete hemolysis of RBCs). Error bars represent the standard error of the mean; ns, non-significant, **** P < 0.00001.
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In vitro cytotoxicity of ibipinabant against: ( A ) Vero and ( B ) ME-180 cells. Results are shown as a percentage of cell viability relative to the negative control (DMSO). ( C ) Hemolytic activity of ibipinabant against human <t>RBCs.</t> The results are shown as a percentage of RBC hemolysis for each concentration of ibipinabant relative to 0.1% Triton X-100 (TX-100; positive control with complete hemolysis of RBCs). Error bars represent the standard error of the mean; ns, non-significant, **** P < 0.00001.
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In vitro cytotoxicity of ibipinabant against: ( A ) Vero and ( B ) ME-180 cells. Results are shown as a percentage of cell viability relative to the negative control (DMSO). ( C ) Hemolytic activity of ibipinabant against human <t>RBCs.</t> The results are shown as a percentage of RBC hemolysis for each concentration of ibipinabant relative to 0.1% Triton X-100 (TX-100; positive control with complete hemolysis of RBCs). Error bars represent the standard error of the mean; ns, non-significant, **** P < 0.00001.
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In vitro cytotoxicity of ibipinabant against: ( A ) Vero and ( B ) ME-180 cells. Results are shown as a percentage of cell viability relative to the negative control (DMSO). ( C ) Hemolytic activity of ibipinabant against human RBCs. The results are shown as a percentage of RBC hemolysis for each concentration of ibipinabant relative to 0.1% Triton X-100 (TX-100; positive control with complete hemolysis of RBCs). Error bars represent the standard error of the mean; ns, non-significant, **** P < 0.00001.

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Identification of the cannabinoid receptor 1 antagonist, ibipinabant, as a potent inhibitor of Neisseria gonorrhoeae

doi: 10.1128/aac.01231-25

Figure Lengend Snippet: In vitro cytotoxicity of ibipinabant against: ( A ) Vero and ( B ) ME-180 cells. Results are shown as a percentage of cell viability relative to the negative control (DMSO). ( C ) Hemolytic activity of ibipinabant against human RBCs. The results are shown as a percentage of RBC hemolysis for each concentration of ibipinabant relative to 0.1% Triton X-100 (TX-100; positive control with complete hemolysis of RBCs). Error bars represent the standard error of the mean; ns, non-significant, **** P < 0.00001.

Article Snippet: Single-donor human RBCs (Innovative Research, MI, USA) were suspended in PBS at a concentration of 4% v/v.

Techniques: In Vitro, Negative Control, Activity Assay, Concentration Assay, Positive Control